Description
Application A proprietary unsymmetrical cyanine dye which is an ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels. SYBR Green I can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower （approximay 100 to 300 pg per band using 254 nm epi-illumination）. SYBR Green II is recommended for ss-DNA and RNA.
SYBR Green I stain has been shown to be much less mutagenic than ethidium bromide in Ames tests. SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide. Staining agarose gels with SYBR Green I does not interfere with the transfer of nucleic acids to membranes or subsequent hybridization in Southern or Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.
Legal Information U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.
  SYBR is a registered trademark of Molecular Probes, Inc.

Properties
usage  mL sufficient for 100 mini-gels
concentration 10,000 × in DMSO
storage temp. −20°C

Safety
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator （EU）, half-mask respirator （US）, multi-purpose combination respirator cartridge （US）
WGK Germany 1
Flash Point（F） 201 °F
Flash Point（C） 94 °C

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