【文獻標題】Treatment of corn with lactic acid or hydrochloric acid modulates the rumen and plasma metabolic profiles as well as inflammatory responses in beef steers

【作者】You Yang、Guozhong Dong、 Zhi Wang、,Junhui Liu et.al

【作者單位】 Southwest University（西南大學）

【文獻中引用產(chǎn)品】

牛白細胞介素1β（IL-1β）ELISA試劑盒

牛白細胞介素6(IL-6)ELISA試劑盒

牛白細胞介素8（IL-8）ELISA試劑盒

牛腫瘤壞死因子α（TNF-α）ELISA試劑盒

【關鍵詞】Metabolomics, Beef steers, Lactic acid, Hydrochloric acid, Corn

【DOI】10.1186/s12917-018-1734-3

【影響因子(IF)】2.6

【出版期刊】《BMC Veterinary Research 》

【產(chǎn)品原文引用】

The LPS concentrations in the rumen fluid and plasma;the concentrations of acute phase proteins in plasma,including C-reactive protein (CRP), serum amyloid A(SAA), haptoglobin (Hp), and LPS-binding protein(LBP); and plasma cytokines concentrations, including interleukin (IL)-1β, IL-6, IL-8 and tumour necrosis  factor-alpha (TNF-α), were determined using a commercially available bovine ELISA kit. A bovine LPS  ELISA kit was purchased from Shanghai Preferred Biotechnology Co., Ltd. (Shanghai, China). Bovine SAA,CRP, Hp and LBP ELISA kits were purchased from Nanjing Jianchen Saihao Biotechnology Co., Ltd. (Nanjing, China). Bovine IL-1β, IL-6, IL-8 and TNF-α ELISA  kits were purchased from Shanghai Jinma Biotechnology Co., Ltd. (Shanghai, China). According to the manufacturer’s instructions, all samples were tested in  duplicate, and the optical density values were read at  450 nm on an automatic microplate reader (EIX808IU;Biotek, Winooski, VT).

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