【文獻標題】Effects of group size on the behaviour, heart rate, immunity, and growth of Holstein dairy calves 

【作者】Jing Lv, Xun-Wu Zhao, Hang Su

【作者單位】東北農(nóng)業(yè)大學（Northeast Agricultural University）

【文獻中引用產(chǎn)品】

牛分泌型免疫球蛋白A(sIgA)ELISA試劑盒

牛白細胞介素2（IL-2）ELISA試劑盒

牛白細胞介素6(IL-6)ELISA試劑盒

牛腫瘤壞死因子α（TNF-α）ELISA試劑盒

【關鍵詞】Calf Group size Behaviour Immunity Growth Animal welfare 

【DOI】doi.org/10.1016/j.applanim.2021.105378 

【影響因子(IF)】4.2

【出版期刊】《APPLIED ANIMAL BEHAVIOUR SCIENCE》

【產(chǎn)品原文引用】The contents of SIgA (E-78235) in saliva and serum IL-2 (E-80069), IL-6 (E80071), and TNF-α (E-80095) were determined using commer cial ELISA test kits (Shanghai Jinma Laboratory Equipment Corpora tion., Ltd., Shanghai, China, LOT201410) following the manufacturer’sinstructions. Briefly, captured antibodies against bovine SIgA or bovine IL-2, IL-6, and TNF-α were coated on plate wells. The serum and saliva samples were incubated with the solution and then washed to remove nonspecific binding. Horseradish peroxidase bovine anti-bovine SIgA or IL-2, IL-6, and TNF-α were used as detection antibodies. Optical density was recorded at 450 nm with a microplate reader (Bio-tek Instrument Inc., USA). Standard curves drawn from the concentration and standard absorbance were used to quantify the saliva SIgA and serum IL-2, IL-6, and TNF-α levels.

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